Anonymous asked in Science & MathematicsChemistry · 8 months ago

Radioactivity labeling question?


determine the number of receptors in a membrane preparation specific for ligand X which is radioactively labelled. Suppose that the specific activity of the radioligand is 2.5 x 1012 cpm per milllimole and the Bmax (maximal binding capacity) is 1.3 x 104 cpm per milligram of membrane protein. There are 1010 cells per milligram of membrane protein. Assume that one ligand binds per receptor. Calculate the number of receptor molecules present per cell. 

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